This page is intended to serve as a record of various terms and histology definitions used at various places/posts within the Atlas.
Alcian Blue/Periodic Acid Schiff (AB/PAS) – a method of staining that identifies mucus by staining it dark purple and epithelial cells magenta. It also stains the nuclei light blue.
Disease progression –
- Control – NOR (Non-obese diabetes resistant) mice that will not develop diabetes
- Non-diabetic – NOD (Non-obese diabetic) mice that did not develop diabetes by 32 weeks
- Early – developed diabetes before or at 20 weeks of age
- Late – developed diabetes at or after 21 weeks of age
Fluorescence in situ hybridization (FISH) – a method of staining that uses fluorescent probes to identify specific cells by binding to a specific part of a nucleic acid sequence.
Hematoxylin & eosin (H&E) – a method of staining that uses the dyes hematoxylin and eosin; hematoxylin stains the ribosomes blue-purple and eosin stains the cytoplasm, collagen, and other structural components.
Immunohistochemistry (IHC) – A common method of immunostaining for the identification of proteins in a section of tissue. Primary antibodies bind to the protein of interest, and secondary antibodies are in turn bound to the original primary. The secondary antibody is often generated with a dye or fluorophore for detection.
Immunohistofluorescence (IHF) – Specific term referring to the use of immunostaining with a fluorescent marker for detection.
Magnification – The resolution of the images when they were captured. Not to be confused with the zoom on the webpage.